TUNEYALI Library (#217744)

Depositing Lab : Irina Borodina

이 CRISPR 기반 library는 Yarrowia lipolytica의 56개 transcription factors를 표적으로 하도록 설계되었으며,이 전사인자들의 발현을 제거(knockout)하거나 조절(modulation)할 수 있게 해줍니다. 이 library는 각 transcription factor의 native promoter)를 서로 다른 강도의 alternative promoters로 교체함으로써, Y. lipolytica 내에서 해당 transcription factor의 발현 수준을 정밀하게 제어할 수 있도록 합니다. 따라서 해당 library는 knockout, activation, inhibition 등의 목적으로 활용될 수 있습니다.

Vector Backbone

pCfB3405 (Plasmid #106166) - does not express Cas9

Publication

Regulating transcription factors in Yarrowia lipolytica (unpublished)

Library Details

Species : Yarrowia lipolytica

Genes targeted : 56 transcription factors

gRNAs : 1 gRNA per gene

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

Volume : ∼20 µL

Concentration : 25 ng/µL

Resource Information

Protocols :

Depositor Data :

Depositor Comments

Figure 1: A CRISPR-Cas9-based method, TUNEYALI, designed for high-throughput gene expression tuning in the widely used industrial yeast Y. lipolytica. The TUNEYALI library facilitates the optimization of Y. lipolytica by targeting 56 transcription factors (TFs) and modulating their expression levels. Using a high-throughput screening platform, strains with optimal performance can be efficiently identified.