


Highlights
•자동
정량화 라이브러리 제작 : Zymo Research의 Sampler
키트에
포함된 “Quick-16S/ITS™
Plus” 반응은
시료 투입량에 상관없이 자동으로 정량화된
라이브러리를
생성하며, 박테리아 16S 유전자
여러 영역과 균류 ITS2 영역을 타겟
합니다.
•PacBio®
& Oxford
장비
호환 : 동일 Sampler에는 “Quick-16S™
Full-Length Library Prep Kit” 반응도 포함되어 있어, PacBio 및 Oxford
Nanopore
Technologies (ONT) 같은
롱리드
플랫폼에서 더 높은 분류학적 해상도를 제공합니다.
•자동화 &
대량
멀티플렉싱
지원
: 단 한 번의 PCR 단계만으로
자동화가 가능하며, 최대 96,
384 혹은 768개 시료의
멀티플렉싱을
지원합니다.
Specifications
•Quick-16S/ITS™
Sampler
Premix Plate Layout
•Sample
Input :
Purified
microbial DNA (≤100 ng), free of PCR inhibitors.
•Primer Sequences and Library Info : For
more details including primer barcodes and sample sheets, visit the respective
kit protocols/product pages directly:

•Amplicon Sizes
:
V3-V4: ~606 bp
ITS:
~480 bp
V4:
~388 bp
V1-V2:
~492 bp
V1-V3:
~650 bp
Full-Length 16S:
~1500 bp
•Equipment Needed
(user provided) : Microcentrifuge,
plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR
Green compatible, recommended), or standard PCR system, and 96-well real-time
PCR plates.
•Index
Primers : Unique
dual
indexes (barcodes) are used to label individual samples for all Quick-16S/ITSTM
Plus reactions while combinational indexes are used for the Quick-16STM
Full-Length Library Prep Kit.
•Barcode
Sequences
: 10bp barcodes
are used for all provided reactions.
•Sequencing
Platform
: Sequencing
of
V1-V2, V1-V3, V3-V4, and fungal ITS2 libraries require an Illumina Miseq/NextSeq.
Sequencing of V4 libraries is possible on all Illumina platforms (with the use
of custom sequencing primers). Full-length 16S libraries are compatible with
both PacBio
& Oxford Nanopore
instruments.