UMI-sgRNA Pooled Libraries

(Pooled Libraries #234819, #234820)

Depositing Lab : Benjamin Ebert

이 라이브러리는 마우스에서 Transcription Factors(TF), 또는 Chromatin Factors(CH)를 표적으로 하는 UMI(Unique Molecular Identifier)-연결 sgRNA 라이브러리입니다.

각 sgRNA는 다수의 UMI와 연결되어 있어, 각각의 렌티바이러스 감염에 대한 클론 단위 추적이 가능합니다.

본 라이브러리는 TF와 CH 인자를 동시에 타겟하기 위해 서로 조합하여 사용할 수도 있습니다.

Vector Backbone

CROPseq-Guide-Puro (Plasmid #86708) - does not express Cas9

Publication

Loke, Kim et al. Blood. 2025 Jun 10; blood.2024028033. doi: 10.1182/blood.2024028033

Library Details

Species : Mouse

Genes targeted : 2,055

gRNAs : 12,727 (6 per element)

Controls : 354

Lentiviral generation : 3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

Volume : ∼15 µL

Concentration : 25 ng/µL

Resource Information

Protocols :

Library Amplification and Sequencing Protocols (DOCX, 22 KB)

Depositor Data :

Library Distribution (PDF, 114 KB)

List of sgRNA Sequences (TF) (CSV, 315 KB)

List of sgRNA Sequences (CH) (CSV, 223 KB)

Unique Molecular Identifier Structure (XLSX, 27 KB)

Terms and Licenses

Academic/Nonprofit Terms :

UBMTA

Industry Terms

Not Available to Industry

Trademarks

Zeocin® is an InvivoGen trademark

Depositor Comments

Given the complexity of UMI-labeled gRNA library, we cannot guarantee that the diversity of the UMI-sgRNA combination will be maintained through multiple subsequent amplifications from this cloned pool.